RESUMO
An astroglial cell line was established from the brain of half smooth tongue sole (Cynoglossus semilaevis) and was designated as CSAC. CSAC shows the morphological homogeneity of epithelial cells. The cell identity was tested by the presence of glial fibrillary acidic protein (GFAP), which was revealed by RT-PCR and immunofluorescence. The cell line was optimally maintained at 24 °C in minimum essential medium supplemented with HEPES, antibiotics, 20% fetal bovine serum, 2-Mercaptoethanol (2-Me) and basic fibroblast growth factor. Chromosome analysis revealed that the CSAC cells maintained a normal diploid chromosome number (2n=42). The fluorescent signals were observed in CSAC after the cells were transfected with green fluorescent protein (GFP) reporter plasmids. The CSAC cell line may serve as a valuable tool for studies on the potential functions of fish astroglial cells.
Assuntos
Astrócitos/citologia , Encéfalo/citologia , Técnicas Citológicas/métodos , Linguados/genética , Animais , Bovinos , Técnicas de Cultura de Células , Linhagem Celular , Cromossomos/genética , Proteína Glial Fibrilar Ácida/genética , Proteínas de Fluorescência Verde/genética , TransfecçãoRESUMO
Over the past few decades, Medaka (Oryzias latipes) has become a model animal in teleost species research due both to its short reproductive cycle and efficient proliferate capacity. Unfortunately, however, systematic data of its sexual differentiation and development have yet to be obtained. In the present study, we observed Medaka gonad development from the earliest recognizable stages through differentiation to maturation under a light microscope, after paraffin sectioning and hematoxylin-eosin staining. The results showed that among juveniles aged 5 to 10 days, the gonad located on the right side of the dorsal abdominal cavity and the germ cells was significantly larger than the surrounding somatic cells. Ten days after hatching, sexual differences between females and males became obvious, and germline cysts derived from oogonium division were present in the gonad. At 50 days after hatching, ovarian cavities and mature sperm were observed. Moreover, during the study we also observed the coexistence of two sexual characteristics during a male's development. Together, these observations fill current gaps in developmental and genetic biology that will allow a more efficient use of Medaka as an experimental model.
Assuntos
Gônadas/crescimento & desenvolvimento , Oryzias/crescimento & desenvolvimento , Diferenciação Sexual , Animais , Feminino , Células Germinativas/crescimento & desenvolvimento , MasculinoRESUMO
As a highly efficient, convenient and nondestructive detection method, near-infrared spectroscopy (NIRS) is appropriate for the analysis of milk and dairy products. The present review discusses the principle of the near-infrared spectroscopy analysis technology and the evaluation standard criteria of milk and dairy products, and also reviews the hardware device technology and the data processing technique for the analysis of milk and dairy products in order to promote the application of near-infrared spectroscopy analysis technology in milk and dairy products quality control in China.
